|
|
| Acesso ao texto completo restrito à biblioteca da Epagri-Chapecó. Para informações adicionais entre em contato com null. |
Registro Completo |
Biblioteca(s): |
Epagri-Chapecó. |
Data corrente: |
01/11/2000 |
Data da última atualização: |
01/11/2000 |
Autoria: |
MELLO, J.C.; MATOS FILHO, A.M. ; BRAUN, R.L. |
Título: |
Cultura das brassicas. |
Ano de publicação: |
1998 |
Fonte/Imprenta: |
Florianopolis, SC: Epagri, 1998. |
Páginas: |
nao paginado. |
Idioma: |
Português |
Palavras-Chave: |
ADUBACAO; BRASSICA; CALAGEM; CLASSIFICACAO; CLIMA; COLHEITA; DOENCA DE PLANTA; EMBALAGEM; IRRIGACAO; MUDA; OLERICULTURA; PLANTIO; PRAGA DA PLNATA; PRATICA CULTURAL; PRODUCAO; SOLO; VARIEDADE. |
Categoria do assunto: |
-- |
Marc: |
LEADER 00776nam a2200337 a 4500 001 1116693 005 2000-11-01 008 1998 bl uuuu u0uu1 u #d 100 1 $aMELLO, J.C. 245 $aCultura das brassicas. 260 $aFlorianopolis, SC: Epagri$c1998 300 $anao paginado. 653 $aADUBACAO 653 $aBRASSICA 653 $aCALAGEM 653 $aCLASSIFICACAO 653 $aCLIMA 653 $aCOLHEITA 653 $aDOENCA DE PLANTA 653 $aEMBALAGEM 653 $aIRRIGACAO 653 $aMUDA 653 $aOLERICULTURA 653 $aPLANTIO 653 $aPRAGA DA PLNATA 653 $aPRATICA CULTURAL 653 $aPRODUCAO 653 $aSOLO 653 $aVARIEDADE 700 1 $aMATOS FILHO, A.M. 700 1 $aBRAUN, R.L.
Download
Esconder MarcMostrar Marc Completo |
Registro original: |
Epagri-Chapecó (Epagri-Chapecó) |
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
|
Voltar
|
|
Registro Completo
Biblioteca(s): |
Epagri-Sede. |
Data corrente: |
27/03/2012 |
Data da última atualização: |
27/03/2012 |
Tipo da produção científica: |
Resumo em Anais de Congresso |
Circulação/Nível: |
-- - -- |
Autoria: |
DALAGNOL, G. L.; MARIOT, A.; BROSE, E.; REIS, M. S.; NODARI, R. O. |
Afiliação: |
Epagri |
Título: |
Genetic characterization of Bromus auleticus Trinius accesses through allozymic and RAPDs markers. |
Ano de publicação: |
2000 |
Fonte/Imprenta: |
In: CONGRESSO NACIONAL DE GENÉTICA, 46., 2000, Aguas de Lindoia, SP. [Proceedings...]. Ribeirao Preto, SP: Sociedade Brasileira de Genetica, 2000. p. 538-539. |
ISSN: |
1415-4757 |
Idioma: |
Inglês |
Conteúdo: |
The natural fields of the South of Brazil come along the years. serving as alimentary base for the cattle. However, this natural resource are not appropriately managed for its better conservation. The Bromus aufeticus Trinius is a perennial native species that is part of this ecosystem and it has been showing potential for good forage and quality. It is a characteristic plant of the Southern plateau of Brazil, with wide, but discontinuous dispersion, with few indívíduos and seemingly, it adapts in environments with some soil limitations. The improvement of this species could allow its cultivation not only in natural habitats but also in areas already degraded by the agricultural activity or excessive grazzing, providing great forage volume with considerable nutritional value. The objective of this work was to characterize the genetic variability among Bromus auleticus accesses existent in the Experimental Station of Lages (EPAGRI S.A.) through of allozymic and RAPD rnarkers.The allozymic analyses were made through elec¬trophoresis in starch gel of maize penetrose (13%), using nine enzymatic systems: PCI, SKDH, IDH, PRX-1, PRX-2, PRX-3, PRX-4, PRX-5, NADHDH, MDH-1, MDH-2, MDH-3, PGM, 6PGDH and a-EST with tris-citrate buffer pH 7.5. The RAPD analysis was accomplished by PCR reactions with 1 U of Taq DNA Polimerase, 1.5 mM of MgCI2, 0.22 dNTPs mM, 0.6 primer mM and 9 ng of genomic DNA, in a final volume of 13 µl. The reactions were submitted to 92oC by one minute, 35oC for one minute, 72oC for two minutes, being this cycle repeated 39 times. One more cycle of 72oC for 5 minutes, for complementation of the reaction were performed afterwards. The enzymatic analysis detected 15 apparent loci with 73.3 % of polymorphic loci and average of 2.1 alleles for loci. The observed heterozigosity was 0.345 and the expected was 0.340. In comparison with other species, the levei found can be considered high. To evaluate the amplification quality and the polymorphism with RAPD markers, 44 primers were tested. From them, 13 (29.5%) amplified visible fragments, revealing 67 bands, 34 of wich were polymorphic. The average of polymorphic bands for each polymorphic primer was 2.61. Eleven Bromus auleticus accesses were evaluated with 7 primers, wich detected 44 bands, of the which 27 were polymorphic. The analyses of genetic similarity were made with BIOSYS-1 and NTSYS programs, using Nei's coefficient (1978) and [accard, for isozymes and RAPD, respectively. In the dendrogram similar genetic groups can be distinguished. RAPDs markers, when compared with isozymes presented larger capacity of polymorphism detection. The high level of genetic variability exhibited by the accesses and it characterization will facilitate the parentaI choosing for the species improvement program, with maintenance of a wide genetic base. Support financial: EPAGRI S. A. /UFSC. MenosThe natural fields of the South of Brazil come along the years. serving as alimentary base for the cattle. However, this natural resource are not appropriately managed for its better conservation. The Bromus aufeticus Trinius is a perennial native species that is part of this ecosystem and it has been showing potential for good forage and quality. It is a characteristic plant of the Southern plateau of Brazil, with wide, but discontinuous dispersion, with few indívíduos and seemingly, it adapts in environments with some soil limitations. The improvement of this species could allow its cultivation not only in natural habitats but also in areas already degraded by the agricultural activity or excessive grazzing, providing great forage volume with considerable nutritional value. The objective of this work was to characterize the genetic variability among Bromus auleticus accesses existent in the Experimental Station of Lages (EPAGRI S.A.) through of allozymic and RAPD rnarkers.The allozymic analyses were made through elec¬trophoresis in starch gel of maize penetrose (13%), using nine enzymatic systems: PCI, SKDH, IDH, PRX-1, PRX-2, PRX-3, PRX-4, PRX-5, NADHDH, MDH-1, MDH-2, MDH-3, PGM, 6PGDH and a-EST with tris-citrate buffer pH 7.5. The RAPD analysis was accomplished by PCR reactions with 1 U of Taq DNA Polimerase, 1.5 mM of MgCI2, 0.22 dNTPs mM, 0.6 primer mM and 9 ng of genomic DNA, in a final volume of 13 µl. The reactions were submitted to 92oC by one minute, 35oC for one ... Mostrar Tudo |
Palavras-Chave: |
Genetic similarity; Heterozigosity; Polymorphic loci. |
Categoria do assunto: |
-- |
|
|
Marc: |
LEADER 03483naa a2200169 a 4500 001 1084130 005 2012-03-27 008 2000 bl uuuu u00u1 u #d 022 $a1415-4757 100 1 $aEpagri 245 $aGenetic characterization of Bromus auleticus Trinius accesses through allozymic and RAPDs markers. 260 $c2000 520 $aThe natural fields of the South of Brazil come along the years. serving as alimentary base for the cattle. However, this natural resource are not appropriately managed for its better conservation. The Bromus aufeticus Trinius is a perennial native species that is part of this ecosystem and it has been showing potential for good forage and quality. It is a characteristic plant of the Southern plateau of Brazil, with wide, but discontinuous dispersion, with few indívíduos and seemingly, it adapts in environments with some soil limitations. The improvement of this species could allow its cultivation not only in natural habitats but also in areas already degraded by the agricultural activity or excessive grazzing, providing great forage volume with considerable nutritional value. The objective of this work was to characterize the genetic variability among Bromus auleticus accesses existent in the Experimental Station of Lages (EPAGRI S.A.) through of allozymic and RAPD rnarkers.The allozymic analyses were made through elec¬trophoresis in starch gel of maize penetrose (13%), using nine enzymatic systems: PCI, SKDH, IDH, PRX-1, PRX-2, PRX-3, PRX-4, PRX-5, NADHDH, MDH-1, MDH-2, MDH-3, PGM, 6PGDH and a-EST with tris-citrate buffer pH 7.5. The RAPD analysis was accomplished by PCR reactions with 1 U of Taq DNA Polimerase, 1.5 mM of MgCI2, 0.22 dNTPs mM, 0.6 primer mM and 9 ng of genomic DNA, in a final volume of 13 µl. The reactions were submitted to 92oC by one minute, 35oC for one minute, 72oC for two minutes, being this cycle repeated 39 times. One more cycle of 72oC for 5 minutes, for complementation of the reaction were performed afterwards. The enzymatic analysis detected 15 apparent loci with 73.3 % of polymorphic loci and average of 2.1 alleles for loci. The observed heterozigosity was 0.345 and the expected was 0.340. In comparison with other species, the levei found can be considered high. To evaluate the amplification quality and the polymorphism with RAPD markers, 44 primers were tested. From them, 13 (29.5%) amplified visible fragments, revealing 67 bands, 34 of wich were polymorphic. The average of polymorphic bands for each polymorphic primer was 2.61. Eleven Bromus auleticus accesses were evaluated with 7 primers, wich detected 44 bands, of the which 27 were polymorphic. The analyses of genetic similarity were made with BIOSYS-1 and NTSYS programs, using Nei's coefficient (1978) and [accard, for isozymes and RAPD, respectively. In the dendrogram similar genetic groups can be distinguished. RAPDs markers, when compared with isozymes presented larger capacity of polymorphism detection. The high level of genetic variability exhibited by the accesses and it characterization will facilitate the parentaI choosing for the species improvement program, with maintenance of a wide genetic base. Support financial: EPAGRI S. A. /UFSC. 653 $aGenetic similarity 653 $aHeterozigosity 653 $aPolymorphic loci 773 $tIn: CONGRESSO NACIONAL DE GENÉTICA, 46., 2000, Aguas de Lindoia, SP. [Proceedings...]. Ribeirao Preto, SP: Sociedade Brasileira de Genetica, 2000. p. 538-539.
Download
Esconder MarcMostrar Marc Completo |
Registro original: |
Epagri-Sede (Epagri-Sede) |
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
Nenhum registro encontrado para a expressão de busca informada. |
|
|